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Biospec Products Inc, Dremel-Racine, WI) in 1.25 ml of iced and freshly prepared 5% (w/v) metaphosphoric acid (Fisher A280100) and centrifuged for 3 min at 7200 g
IGF-1 was demonstrated to regulate MDM2 activity by inhibiting the association between p19ARF and MDM2 in a p38 MAPK-dependent manner (Heron-Milhavet and LeRoith, 2002)
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