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Immunofluorescence staining BMSCs were seeded on coverslips in 24-well plates in -MEM for 24 h and then cultured in osteogenic differentiation medium with different interventions
After treatment, cells were immediately analyzed for apoptosis and the cell cycle using a BD FACSCelesta Cell Analyzer with an Annexin V-FITC/PI Apoptosis Detection Kit and PI/RNase Staining Buffer (BD Biosciences, Franklin Lakes, NJ, USA), respectively, according to the manufacturers instructions
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Food Chem Tox
J., Niessen, F
Nelson et al., 2003
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