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2C and D not only do we observe extreme peak broadening, but in both trials the max absorbance peak wavelength also shifts greater than 80 nm

PMID: 7777652 | doi:10.1016/0306-4530(94)00058-1 Graf MV et al

Compared to control rats, MTX treatment resulted in substantial increases in AST, ALT, and ALP activities, as well as in the concentration of cholesterol, triacylglycerides, and LDL-cholesterol, and a reduction in the serum concentration of HDL-cholesterol

J Biomed Res