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SpragueDawley rats (~ 250 g) were anesthetized by intraperitoneal injection of Beuthanasia-D (38 mg pentobarbital sodium and 6 mg phenytoin sodium/230 g rat) (Schering-Plough Animal Health Corp., Union, NJ)
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The fact that ipamorelin does not activate the cortisol axis at this window makes it pharmacologically well-matched to nighttime administration
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