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This technique is broadly utilized for live-cell tracking of bulk inter-organelle network changes, such as macroscopic mitochondria-lysosome kissing events during autophagy marked by TOMM20 and LAMP1 overlap (55), or broad ER-mitochondria co-localization in microglia (200)
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SLC6A14 relies on the ion gradient on the plasma membrane to drive the intracellular accumulation of amino acids, even under unfavorable amino acid concentration gradients (Nakanishi et al., 2001)
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