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SnCE1 is N-terminally proteolytically processed in eukaryotic cells We expressed full length wild type SnCE1 and the non-acetylated mutants SnCE1 Y212A and SnCE1 C256A with C-terminal myc-His 6 -tag in HEK-293 cells and performed immunoblotting to analyze whether processing occurs from the N- or C-terminus (Fig

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