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Proper handling, including sterile technique and avoiding freeze-thaw cycles, is essential for research reproducibility

Crystals were transferred and soaked in 2 L droplets of crystallization solution for 5 min to ensure sufficient incorporation of cryo-protectant (20% (w/v) PEG 3,350), and subsequently flash frozen in liquid nitrogen for storage

Breaks maintain receptor sensitivity and allow copper levels to normalize

TB-500 (10mg) is a synthetic version of a 43-amino-acid segment of thymosin beta-4, a protein present in most animal cells