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Finally, transfected neurons were immunostained for presynaptic markers, VGLUT1 and synapsin, and postsynaptic marker, PSD-95, to assess functional synapse formation

The use of cell lines in culture offers a consistent and uniform population of cells, reducing variability in results compared to primary cell cultures [77, 78]

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However, it is an invasive, often painful procedure with significant cost and downtime

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BPC-157 is a synthetic peptide derived from a naturally occurring protein found in the stomach