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Incidence and Evolution

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Cell-free supernatants were collected and stored at -20C for the future assays and granulomas were harvested by adding ice-cold sterile 1x PBS to wells, followed by gentle scraping to achieve maximum recovery of granuloma lysates and stored at -20C

Medini et al., 2008

Splitting into twice-daily injections is reasonable at higher daily totals (e.g., 250 mcg AM + 250 mcg PM at a 500 mcg/day total)