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The samples were first lysed in 5% SDS, 9M urea, and 50mM TEAB buffer, sonicated, and centrifuged

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After landing in fourth place on day one with a five-fish limit weighing 15 pounds, 9 ounces, Braaten and Swenson stormed the leaderboard with an identical day two weight for a 31-pound, 2-ounce total