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/ Pull the hook through until the eye is at the nose of the bait, then rotate it 180 degrees so the point faces back toward the body

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brachycephalum Type III AFP of about 7000 Da circulates in the blood at about 20 mg/ml, but this corresponds to an osmotic concentration of just 3 mOsm and can only affect 0.0056 C of freezing point depression equilibrium based on colligative relationships (DeVries and Cheng 2005)