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This can be prevented by derivatizing GSH with the alkylating agent N -ethylmaleimide (NEM) to form GS-NEM before acid deproteination, followed by back-extraction of excess NEM from the deproteinized samples with dichloromethane
Mice deficient in hepsin, a serine protease, exhibit normal embryogenesis and unchanged hepatocyte regeneration ability
Do not continue with the injection if there is blood
This metabolic shift results in increased accumulation and polarization of M2-like macrophages in the TME, contributing to accelerated HCC growth
Official references also describe it as a container designed for repeated entries, which is the key detail behind storage rules and opened-vial dating
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