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Cells were counted and resuspended in proliferation media at a concentration of 100,000 cells per well for 24-well transwells (Corning), and 150,000 cells per 48-well plates

The regulatory relationship between them was further verified using a dual luciferase reporter assay

Bodurtha, MD, MPH (AA, Yale University School of Medicine, 1979) Juliana Kim , Class of 2024, University of Texas Southwestern Medical School Characterizing Fatty Acid Metabolism Alterations in Human BRAF Mutant Melanomas to Identify Novel Therapeutic Vulnerabilities Mentor: Jennifer G

This does not mean regulation is useless

Plasma samples were separated and 200 l was ground into powder in liquid nitrogen

Andres, E