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After washing three times with TBST, membranes were incubated with primary antibodies at 4 overnight
The aortas were then incubated in freshly prepared SA--Gal staining solution at pH 6.0 overnight at 37 C, according to the manufacturers protocol
Over 29 days of treatment, the peptide inhibited pulmonary artery muscularization (the thickening and stiffening of pulmonary arteries that drives this condition), prevented right heart hypertrophy (the enlargement of the right ventricle that occurs when it has to pump against increased resistance), and significantly decreased mortality
[1] [4] Published phase 2 trial data (Jastreboff et al., NEJM , 2023) reported dose ranges from 1 mg up to 12 mg weekly, with higher doses associated with greater weight loss but also increased rates of gastrointestinal side effects
(2023) 11:e1074
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