Sitagliptin and exendin-4 were dissolved in molecular grade distilled water, whereas vildagliptin was initially dissolved in a trace amount of dimethyl sulfoxide (DMSO), and then dilutions were prepared with molecular grade distilled water
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Wu LS, Chang SH, Chang GJ, Liu JR, Chan YH, Lee HF, et al
HSA was immobilized to the surface by pre-activation (1.0 EDC, 1.0 M NHS in PBS, pH 7.4, 7 min) followed by injection of HSA to flow cell 1 (30 g ml 1 in 10 mM NaOAc buffer, pH 5.0, twice for 7 min)
Mangiferin alleviates renal interstitial fibrosis in streptozotocin-induced diabetic mice through regulating the pten/pi3k/akt signaling pathway
H., Jeong E., Kim D