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Add blocking buffer (goat serum) at RT for 1 h

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Unlike oral supplementation, which relies on the digestive system for absorption, IV therapy bypasses the gastrointestinal tract, allowing for quicker and more efficient absorption of nutrients

This dual approach addresses both insulin resistance and beta-cell insufficiency, though individual responses depend on baseline metabolic dysfunction severity and genetic factors influencing each pathway

Sepsis is a severe condition characterized by systemic inflammation, metabolic disturbances, oxidative stress, endothelial dysfunction, and ultimately, poor tissue perfusion

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