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Researchers considering this stack should start with conservative doses of each compound and monitor carefully for unexpected effects

GFP signals within the inner autophagosome membrane are degraded in the autolysosomes but mRFP signals are relatively stable and thus provide a visible measure of autophagic flux status 33

Outflow fractions were collected every 10 min and measured using a glucose/glucose oxidase kit in which lactate oxidase was used to replace glucose oxidase

Diabetes Care (2012) 35:122531

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