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This approach allows for tolerance assessment and reduces the likelihood of adverse effects

Overall, you want to find something with a good balance of electrolytes, sugar, and carbohydrates

The next day, samples were washed in dig wash buffer (wash buffer with 0.02% digitonin) and incubated in dig wash buffer containing pA-MNase (1:1000 dilution) for 1.5 h at 4 C

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Once unfolded, peptide chains can stick to each other and form clumps

Treating earlier risks chasing a moving target, and treating later means you spend months looking older than you should