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iTaq universal SYBR Green supermix (Bio-Rad, #1725124) was used for the reaction in a total volume of 12 l (6 l PCR supermix, 1 l cDNA, 1 l primer, 4 l H 2 O)

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10.1016/0009-8981(75)90055-8 Clin Chim Acta

doi: 10.1152/physrev.00032.2020 3 PatniNGargA

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