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GSH and GSSG concentrations within florets were measured spectrophotometrically with a glutathione reductase (GR)-coupled assay that reduces 5,5-dithio-bis-(2-nitrobenzoic acid) to 5-thio-2-nitrobenzoic acid (Queval and Noctor, 2007)
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A.PivaT
The take home: Individuals who are depleted in glutathione are more prone to oxidative stress (aka free radical damage) and immune dysregulation
Expression of both the PPAT and PAICS genes is coordinately regulated
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