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Carbonyls were derivatised with 2,4-dinitrophenyl hydrazine (Sigma-Aldrich) and coated on an ELISA plate (Nunc-Immuno plate maxisorp, Nunc, Roskilde, Denmark)
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The cyclic structure and the D-residue introduce their own verification questions: mass spectrometry confirms composition but cannot distinguish a D-phenylalanine from an L, since the two are isobaric, and cyclization changes mass by only the loss of water relative to the linear precursor
9(4): p
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