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Use sterile water when: - Preparing single-use vials for immediate use - Diluting further into buffered media shortly after reconstitution - Working with cell culture systems where salt content is controlled downstream Option 2: Bacteriostatic Water (For Multi-Use Vials) Bacteriostatic water contains 0.9% benzyl alcohol as a preservative, which inhibits microbial growth in reconstituted solutions that will be accessed multiple times