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In addition, the characterized LASSS treatment did not stimulate autolysis of HGF as monitored by SDS-PAGE for degradation of 60-kDa -chain and 90-kDa proform of recombinant HGF incubated with LASSS (at 1:8000) at 25 for 2 h (see supplementary Figure 1b), providing another important basis for the availability of LASSS-HGF
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CCK functions in an autocrine manner by binding to the cholecystokinin type A receptor (CCK-AR), inhibiting channel activation and increasing intracellular Ca2+ by up to 30% upon TAS2R stimulation (73)
New delivery mechanisms, such as microneedle patches, sustained release implants, and targeted liposomal carriers, are being developed to enhance tissue targeting and minimize dosing frequency
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