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Animals were euthanized and then cervical vertebrae were dislocated under confirmed deep anesthesia, and tumors were harvested for further study

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A no-template control (NTC) using sterile nuclease-free water (ddH2O) was included to ensure the accuracy of expression quantification, which was performed in duplicate for each sample

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However, following the cumulative addition of BPC 157 up to 10 or 100 g/ml, the vasorelaxation increased to 28.3 3.5% or 48.3 3.2%, respectively