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In previously published protocols different types of derivatization reagents (CMQT or CMLT), different pH of the buffer used in the protocols (7.4) and shorter time of sample reduction and derivatization (3 min) were applied (Tables 3 and 4.) Changing the conditions of reduction and derivatization increases the efficiency of this process, which may give higher final concentrations of the analytes

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Hernndez, F., Menndez, S., Gmez, M., Eng, L., Efecto de la ozonoterapiaintravascular sobre el sistema de la glutation peroxidasa, Rev