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Sodium-proton exchanger activity was determined in cells loaded with the fluorescent cytosolic pH indicator 2'7'-biscarboxyethyl-5,6-carboxyfluorescein acetoxymethylester (BCECF) after pretreatment with 250 nM of the phorbol ester 12-O-tetradecanoylphorbol-13-acetate for 10 min

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As a diluent, saline is sometimes chosen over bacteriostatic water for peptides or compounds that are sensitive to preservatives, since the benzyl alcohol in bacteriostatic water can affect the stability of certain substances

Vitamin C, in turn, helps protect cells against oxidative stress