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For fluorescence staining, we incubated samples for 2 h with Alexa-488 and Alexa-594 conjugated donkey antibodies (1:200) against mouse and rabbit immunoglobulins (Supplementary Figs 6 and 7 are representative images from two sets of immunolabelled cultures of NPCs and neurons)
This assay has been validated for human whole blood, serum, EDTA and heparin plasma, urine, and isolated erythrocytes
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19 Reductive synthesis NADPH is also a crucial electron source for several reductive synthesis reactions, including fatty acids, amino acids, nucleotides, and steroids synthesis to sustain rapid tumor cell growth
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