The initial elution was isocratic with 30% acetonitrile for 2 min, followed by a linear gradient for 15 min up to 100% acetonitrile
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published in the Journal of Applied Physiology confirmed that BPC-157 significantly accelerated outgrowth of Achilles tendon explants, markedly increased in vitro tendon fibroblast migration in a dose-dependent manner, and substantially increased fibroblast survival under HO-induced oxidative stress establishing a three-part cellular mechanism (outgrowth, migration, survival) that distinguishes BPC-157s tendon repair profile from direct mitogenic agents
Anna Mapp, a chemist at the University of Michigan and president of the American Peptide Society, reviewed his work and put it bluntly: "There was no demonstration that I could tell that it was a single pure isolated substance" [17][18][19]