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doi: 10.1248/bpb.b15-01020, 144
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The assay was performed in a plate 96 wells Sterilin (Fisher Scientific, UK) where 10 L of homogenate was transferred to each well containing 180 L of 0.1 M buffer sodium phosphate pH 8.0, 1 mM EDTA, 10 L of 10 mM 5,5-dithiobis (2-nitrobenzoic acid) (DTNB)
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repletion reverses hematologic symptoms within days and neurologic sequelae over weeks, reflecting differential tissue turnover
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