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Wang, W., Z

Western Blot HepG2 cells were homogenized on ice with RIPA buffer supplemented with phosphatase and protease inhibitors (Pierce, Thermo Scientific, MA, USA)

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The membranes were blocked with Protein Free Rapid Blocking Buffer (Shanghai Epizyme Biomedical Technology Co., Ltd, Shanghai, China) for 20 min and incubated with the primary antibodies at 4 C overnight

At a certain point, increasing concentration doesnt improve results

Integrative Peptides occupies the middle ground