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Cell proliferation assay The viability of GC-1 spg, TM3 Leydig, and TM4 Sertoli cells was measured using an MTT assay EZ-Cytox kit (Daeil Lab Services Co., Seoul, Korea) according to the manufacturers instructions Each cell was seeded in 24-well plates at a density of 0.5 10 5 cells/well and incubated at 37 C for 12 h

15 GLP-1RAs also improve -cell proliferation and inhibit -cell apoptosis to promote cellular survival by preventing oxidative stress and lipotoxicity

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minor swelling may occur Visible Results Most see improvement within 12 weeks Sessions may be done every 1014 days depending on your treatment plan