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Studies of this kind must be regarded as tentative, however, as there were not adequate controls in the study including a lack of randomization, double-blinding, or placebo

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The resulting cells were fixed in 4% paraformaldehyde and incubated with a blocking buffer for 1 h, and the cells were permeabilized with 0.2% Triton and 0.1% Tween 20 in a blocking buffer (3% bovine serum albumin in PBS) for 2 h

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Key benefits and findings include: Antioxidant Action: PLE reduces oxidative stress, a critical factor in melasma, by neutralizing free radicals and minimizing UV-induced inflammation