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For experiments in 384-well plates, wells were aspirated, rinsed with PBS and incubated with a fixative and staining solution containing 3.7% formaldehyde (Fisher Scientific, F75P-1GAL) and 5 g ml 1 Hoechst 33342, trihydrochloride, trihydrate (Invitrogen, H1399)

All patients complete a brief medical intake form before their appointment, and our physician-supervised medical team reviews it to confirm the treatment is appropriate

Changes in Th9 and Th17 lymphocytes and functional cytokines and their relationship with thyroid-stimulating hormone receptor antibodies at different stages of graves' disease

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Schneider R et al

Only for the brief minutes needed to withdraw a dose