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Whole-cell patch-clamp recordings were performed on mRFP--actin-transfected hippocampal neurons to measure miniature excitatory postsynaptic currents (mEPSCs), reflecting synaptic activity [1]

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For deficiency, weekly injections are common during the loading phase, and monthly maintenance is standard long-term

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In total, these efforts yielded an atlas of 301,268 glial and 229,129 neurons (Supplementary Table 3), clustering into 10 glial cell types and 80 neuronal populations with unique marker genes (Fig

At first, higher doses are used to quickly fill up vitamin B12 stores