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Lyophilized peptides must be stored at 20C, and once reconstituted with bacteriostatic water, refrigeration at 28C maintains stability for approximately 28 days
However, if the goal is to study systemic effectslike tendon repair, muscle recovery, or neuroprotectiona higher dose might be necessary to ensure enough of the compound survives the digestive gauntlet and reaches the bloodstream to circulate throughout the body
Most people meet these needs through diet alone, but older adults, people on strict plant-based diets, and those with digestive disorders may benefit from supplements, though dosages vary based on individual needs
Only the lyophilized powder should ever be frozen
D., Crocetti, D., Tommerdahl, M., Mostofsky, S
2.9 Cell culture and treatment Mouse colon epithelial cells (MCECs) were cultured in high-glucose Dulbeccos Modified Eagle Medium, supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (P/S), in a humidified incubator with 5% CO 2 at 37C
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