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To move beyond basic cell culture, the study incorporated ex vivo skin models

At specified time intervals (0 h, 1 h, 2 h, 4 h, 6 h, 8 h, 12 h, 24 h, 48 h), the sample solution (1.5 mL) was withdrawn from the dialysate, after which an equal volume of fresh corresponding solution (1.5 mL) was promptly added back into the dialysate to maintain the overall volume

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Our data profoundly contribute to the decoding of PPO activity and specifies the amino acid positions that direct mono- and diphenolase activity

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