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After incubation on ice for 1 h, the homogenates were centrifuged at 20,400 g for 20 min at 4 C, and the resulting supernatants were appropriately diluted and used in the assays

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In that case, stop using the product right away (if its a topical, gently wash it off with cool water) and seek prompt medical attention

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Additionally, iNOS is among the central downstream genes of NF-B, but, in turn, iNOS can promote and inhibit NF-B activity (142)