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For viability, 7-aminoactinomycin D (7AAD) (Thermo fisher A1310) or Zombie NIR (BioLegend 423106) diluted 1:3000 was stained concurrently with antibodies in all flow cytometry assays
Injection training: patients receive hands-on training on subcutaneous self-injection technique, vial storage (refrigerated), and reconstitution if supplied lyophilized
About HPLC Testing: High-Performance Liquid Chromatography (HPLC) is a standard analytical technique used to verify peptide purity
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