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Utilizing Light Cycler 480, SYBR Premix Ex Taq TM II, and gene-specific primer pairs, quantitative real-time PCR was carried out using generated cDNAs as templates (Roche)

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By linking ageing and oocyte quality decline, we will continue to explore ways to address the age-related decline in oocyte capacity and overcome the effects of mitochondrial dysfunction in the future

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