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According to the cited figure 3[14], the increase of NADPH concentration from 2,5 mM [1] up to 10 mM also increases the velocity of GR and the GSH reserve levels, that are increased from 10 mM up to 100 mM, in such a way to always saturate GP activity

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The supernatant of HEK293 cells enriched with glycosylated proIGF-1Ea, proIGF-1Eb, proIGF-1Ec and mature IGF-1 were digested with trypsin, loaded on SDS-PAGE gels and probed with the anti-mature IGF-1 antibody

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