The time lapse contains examples (white arrowheads) of microglia (green) comprising honeycomb networks that collapse and immediately fill the holes in their network likely as a result of astrocyte cell death in the glial limitans
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This agrees with previous research reports, which showed that reduced solubility during heating is associated with enhanced protein aggregation caused by molecular unfolding (Chen et al., 2018)
Nature Neuroscience, 27 (3), 433448