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Untreated tissues in 2% FBS DMEM were used as negative controls

In glutathione metabolism, 2 molecules of GSH are oxidized by GSH peroxidase to one molecule of GSSG to directly scavenge ROS [26], while glutathione reductase (GR) converts GSSG to 2 molecules of GSH primarily using nicotinamide adenine dinucleotide phosphate (NADPH) or, in rare cases, NADH [reduced form of nicotinamide adenine dinucleotide (oxidized form) (NAD + )] [27]