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IGF1R is linked to various cytoplasmic second messenger molecules including the RAS-MAPK and PI3K signaling networks

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Formation of hydrogen peroxide was determined after addition to the solution of 10 l of 2-7dichlorofluorescein diacetate (2m in ethanol) and DCF fluorescence measured as described previously (Garca-Ruiz et al, 1995, 1997)

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