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Identity of synthetic AQ-QI and DEAQ-QI was verified by mass spectrometry and the purities were above 95% (Supplementary Figure S1), as determined by HPLC-UV and LC-TOF-MS ( Cell Culture HepG2 cells were cultured in collagen-coated plates and maintained in DMEM containing 10% FBS, 1% penicillin/streptomycin (PAA Laboratories, Austria), 1% ultraglutamine (Lonza, Switzerland) and 1% non-essential amino acids (Sigma-Aldrich, Germany)

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Toronto [Ont.]: University of Toronto Press

Your cells then use this cysteine, along with glycine and glutamate, to produce and use glutathione

PMID 17298593

Whats methionine