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The fresh sample was extracted from 10% TCA solution, centrifuged 10,000 g , for 10 min under cold condition and the supernatant was incubated in an assay mixture [0.2 M phosphate buffer, 15 mM dithiobis-(2-nitrobenzoic acid)] and diluted with water for 30 min

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The solutions were diluted with methanol/water(1/1, v/v) to achieve concentrations of 1 g/ml of dexketoprofen for plasma analysis and 2 g/ml of dexketoprofen for fecal homogenates, urine and bile analysis

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Treatment should not be delayed if neurological symptoms occur, as nerve damage may become irreversible without prompt B12 replacement