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The technique is based on the reversible interaction between a protein (or a group of proteins) and a specific ligand bound to a chromatographic matrix to separate proteins
10.1016/S0140-6736(89)91909-0 Lancet 23 CaiJ.ChenY.SethS.FurukawaS.CompansR
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These shifts appeared before participants reported feeling different
Animal data on LR3 infusion in pigs showed continued suppression of plasma growth hormone (~23% decrease) and IGFBP-3 across the 4-day infusion window (Tomas et al., 1997) by days 8-10 of a 10-day human community protocol, this kind of HPG-axis suppression would be expected to be active
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