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The mood-based changes may also be explained by indirect effects

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CK: Writing original draft, Writing review & editing

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If your refrigerator has an interior light that stays on, store vials in a non-transparent (opaque) container to block light exposure

The obtained good 2D classes were used for template-based automatic particle picking, and false positives or bad particles were subsequently eliminated over two rounds of 2D classification, resulting in 9101 selected particles