Cells were fixed with 4% PFA for 30 minutes, permeabilized with 0.1% Triton X-100, and blocked with 5% normal goat or donkey serum
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Topical application without delivery enhancement deposits the molecule on the outermost stratum corneum (10 to 20 micrometers thick), which is the wrong tissue for the receptor biology to engage 2
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Supplementary Information Additional file1 (download DOCX : Primers used for PCR Additional file2 (download TIF : KEGG pathway results related to cell growth and death and aging. The ferroptosis pathway ranked first Additional file3 (download JPG : Identification of ADSCs