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eWAT explants (500 mg) were digested in 1 mL DMEM supplemented with 2% fatty acidfree BSA (Sigma-Aldrich, 126575), HEPES (10 mM), Liberase TM (thermolysin medium) (25 g/mL) (Roche, 05401119001), and DNAse (250 g/mL) (Roche, 10104159001) for 1 hour at 37C

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