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In some cases, stability may extend beyond two years provided the vial remains sealed

The proteome concentration of the tissue lysates was determined using the DC protein assay (Bio-Rad), normalized to 2 mg/mL, and then 100 L of each sample was transferred to a LoBind Eppendorf tube containing 48 mg of urea

But in all of these cases, patients were taking multiple medications and either had other autoimmune diseases or a strong family history of autoimmune disease